Quantitative, multimodal cell and fiber mapping in full primate brain sections

Detailed multi-modal architecture information is the basis for understanding function, dysfunction, and potential treatment of the brain. Mouse models have led the establishment of new molecular markers and have engendered a rise in understanding cell-specific function. Information based on such markers in the human brain is highly fragmented, and major parts are missing. While cell types often exhibit homology across species, the size and organizational complexity of the human brain make direct inference of function from mouse data problematic. There are multiple efforts to generate complete and consistent maps for various species, but none addresses (quantitative) protein expression in combination with direct imaging of fiber distribution patterns.
We aim to bridge this gap by using our newly developed method to integrate multi-channel, cell-type specific immunohistochemistry with polarized light imaging (3D-PLI), to map protein expression, as well as fiber architecture in 3D-space, in the same, full, primate brain sections.